Fig. 5: DG induces Rim13-dependent necrosis
From: Diacylglycerol triggers Rim101 pathway–dependent necrosis in yeast: a model for lipotoxicity
a Screen for knockout mutants rescuing DOG-induced cell death based on PI staining. b, c Mass spectrometry-assisted quantification of DOG b and total DG c levels. d Clonogenic survival plating and e DHE to ethidium conversion for ROS quantification after DOG treatment comparing wild type to rim13∆. f Representative microscopy pictures of ROS assessment for DOG-treated samples. Scale bar = 5 µm. Clonogenic survival assay g and ROS assessment h comparing TKO rim13∆ to DKO, TKO and wild-type cells. i FACS-assisted analysis of AnnexinV/PI co-staining. Statistical significance in panels a-d was analyzed using two-way ANOVA with strain and treatment as independent factors. Panels b and c were additionally analyzed by one-way ANOVA to assess significant change after DOG treatment. Statistical significance of g, h, and i was determined using one-way ANOVA. Significance in i refers to the PI fraction. Error bars indicate standard error of the mean (SEM) and asterisks in the figures indicate significant differences, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001
