Fig. 6: The Rim101 cascade is involved in DOG-induced cell death

a Clonogenic survival and b DHE to ethidium conversion of rim13∆ upon DOG treatment expressing either the wild-type RIM13 gene or rim13 bearing the C128A point mutation. DHE to ethidium conversion was detected 24 h and survival was determined 25 h after DOG stress. c Clonogenic survival and d DHE to ethidium conversion upon DOG treatment was detected in rim13∆ with expression of carboxy-terminally truncated or full RIM13 on a plasmid. e Clonogenic survival and f DHE to ethidium conversion of rim101∆ upon DOG treatment. g Clonogenic survival of rim13∆ with and without expression of constitutively active Rim101_1-531 upon DOG treatment. ‘EV’ indicates empty vector control. h Clonogenic survival assay and i PI staining were detected in Rim21 sensor complex single deletion mutants rim21∆, rim9∆, dfg16∆, and rim8∆ upon DOG stress j Clonogenic survival assay and the impact of RIM21 deletion in TKO background. PI staining of the same experiment is shown in Supplementary Fig. 5a. Statistical significance was assessed using two-way ANOVA with strain and treatment as independent factors. Error bars indicate SEM and asterisks in the figures indicate significant differences, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001