Fig. 1: Pharmacological modulation of p53 impacts PINK1 transcription.
From: Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation
SH-SY5Y cells were treated with vehicle (CT), etoposide (ETO, 150 µM, 16 h) or pifithrin-alpha (PFT, 10 µM, 16 h) then PINK1 protein (a,d, N = 9), promoter activity (b, N = 9) and mRNA levels (c,e, N = 6) were analyzed as described in the Methods section. p53 and actin immunoreactivities are provided as read-out of p53 activation and gel loading controls (a, d, respectively). Bars represent the means ± SEM of 3 independent experiments performed in triplicate (a, b, d) or duplicates (c,e) and are expressed as percentage of vehicle-treated control cells. Statistical analyses were performed with GraphPad Prism software by using unpaired Student’s t-test. Significant differences are: *p < 0.05, **p < 0.01, ***p < 0.001.
