Fig. 3: p53 controls PINK1 transcription in vivo.
From: Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation
(a,b) Effect of adenovirus-mediated wild-type p53 (Adp53, black bars) overexpression on PINK1 protein (a, N = 8) and mRNA (b, N = 8) levels compared to control virus (AdCt, white bars) in vivo as described in the Methods. Analyses of PINK1 protein (c, N = 6) and mRNA levels (d, N = 8) in control (p53+/+, white bars) or TP53 gene invalidated (p53−/−, black bars) in mouse brain samples as described in the Methods. Representative gels of PINK1, p53 (virus transduction efficiency) and actin (protein charge control) protein levels are provided in upper panels a and c. Bars represent the means ± SEM of 2 independent experiments performed in quadruplicates and are expressed as percentage of control (AdCt, p53+/+) mouse brain samples. Bars represent the means ± SEM of 3 or 4 independent experiments performed in duplicate (c,d, respectively) and are expressed as percentage of control (AdCt, p53+/+) mouse brain samples. Statistical analyses were performed with GraphPad Prism software by using unpaired Student’s t-test. Significant differences are: *p < 0.05, and ***p < 0.001.
