Fig. 8: p53 repression of autophagy is PINK1-dependent.
From: Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation
LC3-2/LC3-1 (a,b, N = 12), p62 (a,c, N = 12), TIM23 (a,d, N = 12), TOM20 (a,e, N = 12) and HSP60 (a,f, N = 12) protein levels were analyzed in control (PINK+/+) or Pink1-deficient (PINK−/−) HAP cells treated with vehicle (Ct), pifithrin-α (PFT, 30 µM, 4 h) or CCCP (CP, 10 µM, 6 h) as described in the Methods. Bars represent the means ± SEM of 4 independent experiments performed in triplicates and are expressed as percent of control HAP1 (HAP+/+, Ct) cells. Actin expression is provided as a representative gel loading control in a. Statistical analyses were performed with GraphPad Prism software by using unpaired Student’s t-test. Significant differences are: ns not significant, *p < 0.05, ** p < 0.01 and ***p < 0.001.
