Fig. 4: Breast cancer cells require MASTL kinase activity

a Immunoblotting analysis of MASTL protein level in iCas9 and isgMASTL cells expressing murine wild-type (WT) or kinase dead (G43S) Mastl forms fused to GFP in the presence and absence of doxycycline (4 days treatment). b FACS profiles showing accumulation of ≥4n cells after depletion of MASTL and rescue by the WT, but not G43S, murine Mastl forms. The graph shows the fold induction in the percentage of ≥4n cells upon doxycycline (Dox) treatment. Bars indicate means + SD for three independent experiments. c Mastl WT, but not G43S, rescues cell growth in colony formation assays. Graph on the left panel shows cell growth quantification, where cell proliferation in the absence of doxycycline was set as 100%. Bars indicate means + SD for three independent experiments. The middle and right panels show the crystal violet staining of one representative experiment. ***, p < 0.001; Student t test. Dox doxycycline