Fig. 5: MASTL knockout impairs growth of tumor cells in vivo

a Growth of isgMASTL MDA-MB-231 xenotransplants either untreated (white) or after continuous treatment with doxycycline-enriched diet (red). * p < 0.05, ***, p < 0.001; 2way ANOVA. b Tumor weight at the endpoint of the xenograft assay shown in a. * p < 0.05; Student t test. c Immunohistochemical analysis of MASTL, Ki67 and Cas9 protein levels in representative tumors of the isgMASTL xenograft assay. d. Histogram showing the percentage of MASTL- and Ki67-positive cells in three representative tumors of each experimental condition. Cas9-positive cells were scored in doxycycline-treated mice. Bars represent means + SD of three random areas per tumor. e Nuclear volume analysis in isgMASTL untreated and doxycycline-treated tumors at the endpoint. Representative images of DAPI staining are shown on the left panel. The graph shows the quantification of three representative tumors of each experimental condition (untreated and continuous doxycycline treatment). ****, p < 0.0001; Mann-Whitney test. Dox doxycycline. Scale bars, 25 µm