Fig. 3: Loss of MLKL does not affect the control and progression of Mtb infection in vivo | Cell Death & Differentiation

Fig. 3: Loss of MLKL does not affect the control and progression of Mtb infection in vivo

From: Necroptotic signaling is primed in Mycobacterium tuberculosis-infected macrophages, but its pathophysiological consequence in disease is restricted

Fig. 3

a–d Wild-type and Mlkl −/ − mice were infected with Mtb by aerosol, and sacrificed at the indicated times post-infection. a Lungs and b spleens were homogenized and plated on 7H11 agar plates to enumerate CFU per organ. Horizontal bars indicate the median and each point represents one mouse. Data were pooled from two (week four and eight) or three (day 10) independent experiments (n = 8–14 per group). There were no statistically significant differences between genotypes (p > 0.05; t test). c Lung histology of mice four weeks post-infection. Sections of the left lobe were stained with H&E. Representative of 12 mice of each genotype. Scale bar represents 1 mm. d Quantitation of H&E-stained lung sections in terms of both the percentage of the total section surface area comprising inflammatory areas (upper), as well as the number of inflammatory areas per section (lower). Graphs show mean and SEM of pooled data from two independent experiments (n = 10 per group). There were no statistically significant differences between genotypes (p > 0.05; Mann–Whitney test)

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