Fig. 4: Infiltration of innate immune cells into the lungs following Mtb infection is not affected by the loss of MLKL signaling | Cell Death & Differentiation

Fig. 4: Infiltration of innate immune cells into the lungs following Mtb infection is not affected by the loss of MLKL signaling

From: Necroptotic signaling is primed in Mycobacterium tuberculosis-infected macrophages, but its pathophysiological consequence in disease is restricted

Fig. 4

a Flow cytometric analysis of the total number of CD11b+Gr1lo macrophages, CD11b+Gr1hi granulocytes and CD11c+MHCII+ dendritic cells isolated from the lungs of mice four weeks after infection. b Immunohistochemical staining for F4/80 in lung sections from wild-type and Mlkl −/ − mice sacrificed four weeks after infection, and c quantitation of the number of F4/80-positive cells per 100,000 cells. Arrows indicate examples of clusters of positive-stained cells. Representative of two independent experiments of n = 3–4 in each group. Scale bar represents 100 μm. d TNF and IL-1β levels quantitated by ELISA in the lung homogenates of mice four weeks after infection. Data were pooled from two independent experiments (n = 9–11 in each group). a, b, d Graphs show mean and SEM. There were no statistically significant differences between genotypes (p > 0.05; t test)

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