Fig. 5: The adaptive immunological response to Mtb infection in vivo is not perturbed by the loss of MLKL signaling | Cell Death & Differentiation

Fig. 5: The adaptive immunological response to Mtb infection in vivo is not perturbed by the loss of MLKL signaling

From: Necroptotic signaling is primed in Mycobacterium tuberculosis-infected macrophages, but its pathophysiological consequence in disease is restricted

Fig. 5

a Flow cytometric analysis of the total number of CD4+ T cells, CD8+ T cells and CD19+MHCII+ B cells isolated from the lungs of mice four weeks after infection. b Immunofluorescence staining (blue DAPI, green CD3 and red CD4) of lung sections from mice four weeks after infection. Representative of n = 4 in each group. Three inflammatory lesions were examined per mouse. Scale bar represents 100 μm. c Quantitation of the number of Mtb ESAT-6-specific CD4+ T cells in the lungs of mice four weeks after infection. Cells were isolated from the lungs and restimulated ex vivo with either ESAT-6 or OVA peptide, and analyzed for TNF and IFNγ production by intracellular cytokine staining and flow cytometry. (left) Representative flow cytometry plots and (middle) quantitation of the total number of TNF+IFNγ+CD4+ T cells and (right) their frequency expressed as a percentage of total CD4+ T cells are shown. a, c Graphs show mean and SEM of pooled data from two independent experiments (n = 6 per group). There were no statistically significant differences between genotypes (p > 0.05; t test)

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