Fig. 3 | Cell Death & Differentiation

Fig. 3

From: Anti-apoptotic A1 is not essential for lymphoma development in Eµ-Myc mice but helps sustain transplanted Eµ-Myc tumour cells

Fig. 3

Loss of A1 does not enhance apoptosis of pre-leukaemic pro/pre-B cells in vitro. a Viability (%) of bone marrow B220+sIg- cells obtained by FACS sorting from healthy (28–30 day-old) A1+/+ Eµ-Myc and A1−/− Eµ-Myc mice (n = 3–6 per genotype) and cultured for 48 h without added cytokines. Viability was measured at 0, 4, 8, 24 and 48 h by flow cytometry after staining with propidium iodide (PI) and Annexin V. Bars represent mean viability (PI- Annexin V−) ± SEM. No significant differences were found between A1+/+ Eµ-Myc (light grey bars) and A1−/− Eµ-Myc (dark grey bars) cells (multiple Student’s T-tests). b Analysis of A1 expression in bone marrow pro/pre-B (B220+sIg−) and splenic B (B220+sIg+) cells obtained by FACS sorting from healthy young A1+/+, A1−/−, A1+/+ Eµ-Myc, and A1−/− Eµ-Myc mice. Cell lysates (15 µg) from independent mice were run in each lane. Data are representative of two independent experiments. Molecular weight markers are indicated (kD). The immature B cell WEHI-231 line was used as a positive control for A1; treatment with cycloheximide (CHX) resulted in loss of A1 due to its short half-life

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