Fig. 5
A1−/− Eµ-Myc haemopoietic stem and progenitor cells are as competitive as A1+/+ Eµ-Myc cells in reconstituting haemopoiesis. Bone marrow competitive reconstitution experiments were set up using UBC-GFP/Eµ-Myc cells as the competitor. For analysis these can be distinguished by GFP expression from the A1+/+ Eµ-Myc and A1−/− Eµ-Myc test bone marrow cells, and by Ly5.2 expression from the Ly5.1 recipients. For each experiment UBC-GFP/Eµ-Myc cells were mixed in a 1:1 ratio with either A1+/+ Eµ-Myc or A1−/− Eµ-Myc cells and a total of 3 × 106 cells transplanted into three lethally irradiated Ly5.1 recipients. Six weeks post reconstitution, mice were bled and analysed for GFP, Ly5.2, B220, IgM, IgD, CD4, CD8, Mac1 and Gr1 expression by flow cytometry. The data shown here represents mean ± SEM of 6 independent experiments. Data plotted is % GFP−of total Ly5.2+, so is a comparison of the test bone marrow vs only UBC-GFP/Eµ-Myc (Ly5.1 cells have been excluded)
