Fig. 4: High glucose treatment upregulates miR-34a in cultured human cardiomyocytes | Cell Death & Differentiation

Fig. 4: High glucose treatment upregulates miR-34a in cultured human cardiomyocytes

From: Diabetes induces the activation of pro-ageing miR-34a in the heart, but has differential effects on cardiomyocytes and cardiac progenitor cells

Fig. 4

a Quantitative scatter plots showing the differential expression of miR-34a by quantitative RT-PCR analysis in the AC-16 human ventricular cardiomyocytes cultured under normal glucose (NG) or high glucose (HG). Equal concentration of mannitol was added to the NG cultured cells as the osmotic control. Data are expressed as Log fold change in miR-34a expression and are mean ± SEM. b, c Representative immunoblot images and quantitative bar graphs showing the expression of SIRT-1 b and p53 c in HG and NG cultured cardiomyocytes. β-actin was used as internal control. Data are represented as fold changes to NG cultured cells and are mean ± SEM. d Quantitative bar graphs showing the caspase-3/7 activity in NG and HG cultured cells. Data are represented as relative luciferase units and are mean ± SEM. *P < 0.05 nd ****P < 0.001 vs. normal glucose (NG) treated cells. All the experiments were performed in triplicates

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