Fig. 7 | Cell Death & Differentiation

Fig. 7

From: Diabetes induces the activation of pro-ageing miR-34a in the heart, but has differential effects on cardiomyocytes and cardiac progenitor cells

Fig. 7

miR-34a activity inhibition is not beneficial in human diabetic CPCs. a, b Representative immunoblot images and quantitative bar graphs showing the expression of SIRT-1 a and p53 b in human CPCs following inhibition of miR-34a (anti-miR-34a) activity. β-actin was used as internal control. Data are represented as fold changes to non-diabetic (ND) CPCs transfected with Scrambled sequence (Scr). Values are mean ± SEM. c Quantitative bar graphs showing the caspase-3/7 activity in AC-16 following inhibition of miR-34a (anti-miR-34a) activity. Data are represented as relative luciferase units and are mean ± SEM. d Quantitative bar graphs showing the number of CPCs at the end of treatment period measured by cyquant assay to determine the proliferation rate following inhibition of miR-34a (anti-miR-34a) activity. Data are represented as cell number and are mean ± SEM. *P < 0.05 and **P < 0.01 vs. ND-Scr CPCs; ## P < 0.01 and ### P < 0.001 vs. corresponding Scrambled sequence (Scr) transfected CPCs. All the experiments were performed in CPCs collected from three different patients

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