Fig. 1

Nrf3 is expressed in basal keratinocytes, but dispensable for skin development and homeostasis. a qRT-PCR of epidermal and dermal RNA for vimentin (Vim), K14 and Nrf3, normalized to Rps29. b qRT-PCR of RNA from basal and suprabasal layers of mouse tail skin for Nrf3. RNAs were pooled from five mice per genotype, two pools were analysed in independent experiments. The efficiency of basal/suprabasal separation in both experiments (Exp1 and Exp2) was verified by analysis of the suprabasal/basal ratio of Sprr2a expression, a marker for differentiated keratinocytes, as indicated. Expression in basal keratinocytes was set to 1. c NRF3 immunofluorescence staining of human skin sections (green), counterstained with DAPI (blue). Bar: 20 μm. d NRF3 immunofluorescence staining of HaCaT keratinocytes (red), counterstained with ER tracker (green) and Hoechst (blue). Note the ER localization of NRF3. e Hematoxylin/eosin (H/E; upper panel) and Ki67 immunohistochemistry staining (lower panel) of sections from back skin of wt and Nrf3-ko mice. Bars: 10 μm (H/E) and 100 μm (Ki67). The indent shows a higher magnification of the area indicated with a rectangle. Quantification of the number of Ki67 positive cells/mm of basement membrane is shown below. f Immunofluorescence staining of back skin sections for involucrin (Inv), K10, K14, or K6 (red), counterstained with DAPI (blue). Bar: 20 μm. Scatter plots in a, e show mean and standard deviation (S.D.). Each data point represents results from an individual mouse