Fig. 5
From: Nrf3 promotes UV-induced keratinocyte apoptosis through suppression of cell adhesion
The effect of Nrf3 on UV-induced apoptosis is independent of ROS and Nrf2. a Flow cytometry analysis of DCF in non-irradiated (-UV) immortalized murine keratinocytes from Nrf3-ko and wt mice and 6 h after irradiation with 10 mJ/cm2 UVB. Relative drift of peaks was measured in two independent experiments with two immortalized keratinocyte lines of each genotype. Results from a representative experiment are shown. b Nrf3-ko, Nrf2-ko, Nrf2-ko/Nrf3-ko, and wt mice were irradiated with 100 mJ/cm2 UVB. Epidermal RNA from skin 24 h after UVB irradiation was analyzed by qRT-PCR for expression of Nqo1, Srxn1, GstA3, and Nrf2 relative to Rps29. Scatter plots show mean and SD. Each data point represents the result from an individual mouse
