Fig. 7
From: Nrf3 promotes UV-induced keratinocyte apoptosis through suppression of cell adhesion
Loss-of-Nrf3 in keratinocytes promotes focal adhesion formation and migration. Immortalized murine keratinocytes were allowed to attach for 24 h on uncoated plates and stained with Alexa Fluor 488-coupled phalloidin and vinculin (a) or zyxin (b) antibodies. Representative cells of the different genotypes are shown. Large FAs in Nrf3-ko cells are indicated by arrowheads (a). Quantification of FAs is shown in the bar graph. All results shown are representatives of at least two independent experiments. c HaCaT keratinocytes were analyzed by NRF3 immunofluorescence. The actin cytoskeleton was stained with Alexa Fluor 488-coupled phalloidin and nuclei were counterstained with Hoechst. Magnification bars: 20 µm (a–c). All results shown are representatives of at least two independent experiments. d–e Immortalized murine keratinocytes were subjected to scratch wounding and analyzed by live cell imaging for 20 h. Five cells from the front row from a minimum of five movies were analyzed for each genotype. All results were reproduced with three independent wt and two Nrf3-ko cell lines in four independent experiments. d Schematic illustration of parameters analyzed for the quantification of cell migration. Migrating immortalized murine keratinocytes were analyzed for velocity (a/time), (b) displacement, (c) perpendicular movement and (d) and persistence coefficient (“b”/”a” in e; [35]. Scatter plots with mean and S.D. are shown. Statistical analysis was performed using 1-way ANOVA with Tukey’s multiple comparison test
