Fig. 3 | Cell Death & Differentiation

Fig. 3

From: GATA4 as a novel regulator involved in the development of the neural crest and craniofacial skeleton via Barx1

Fig. 3

Inactivation of GATA4 in NCCs impairs cell proliferation, migration, and differentiation in vitro. a Cell counting kit-8 assay was used to examine the proliferation of NCCs after infection with GATA4 lentivirus (n = 5). b, c Quantitative analysis of cell death by flow cytometry showed no significant difference between the two groups (n = 5). d Wound scratch assay indicated attenuated migration ability of NCCs at 24 h after GATA4 knockdown; scale bar: 100 μm. e After NCCs mineralization for 14 days, Alizarin Red staining was conducted and observed under microscope; scale bar: 100 μm. f Semi-quantitative estimation of calcium (n = 5). g, h The qRT-PCR assay indicated downregulation of NCCs key genes in the pa1 of embryos at embryonic day 10.5 (E10.5) after GATA4 knockout in NCCs (n = 5). The data are presented as the mean ± S.E.M. from at least three independent experiments. *P < 0.05, **P < 0.01. NCC neural crest cell, ns not significant, pa1 pharyngeal arch 1, qRT-PCR quantitative reverse transcription-polymerase chain reaction

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