Fig. 4

BARX1 is one of the differential proteins identified in NCCs after GATA4 knockdown by iTRAQ assay. a Flow chart of proteomic analysis for identification of differentially expressed proteins between shCTRL and shGATA4 in neural crest cells. b Forty-three proteins were upregulated and 19 proteins were downregulated after GATA4 knockdown in NCCs. c Cluster analyses of 1888 gene product ratios measured at the protein level by iTRAQ analysis. Proteins upregulated over the control group are displayed in red, and the downregulated proteins are shown in green. d A candidate downregulated protein, BARX1, was examined in NCCs using qRT-PCR (n = 5). e BARX1 was also examined in pa1 of mice at E10.5 using qRT-PCR (n = 5). f, g Protein expression of BARX1 in the NCCs was tested by Western blotting (n = 3). The data are presented as the mean ± S.E.M. from at least three independent experiments. ^*P < 0.05, ^**P < 0.01. iTRAQ isobaric tags for relative and absolute quantitation, NCC neural crest cell, qRT-PCR quantitative reverse transcription-polymerase chain reaction, shCTRL short hairpin control (control lentivirus)