Fig. 4 | Cell Death & Differentiation

Fig. 4

From: LncRNA CASC9 promotes esophageal squamous cell carcinoma metastasis through upregulating LAMC2 expression by interacting with the CREB-binding protein

Fig. 4

CASC9 upregulates the expression of genes associated with ECM–receptor interactions and focal adhesion pathways. a, b Western blot and IF indicates the expression of LAMC2 and ITGB4 after CASC9 knockdown using two siRNAs in KYSE150 and KYSE450 cells. Original magnification, ×400. Scale bars, 40 µm. c Fold change in LAMC2 expression between ESCC tissues and normal tissues normalized by log2 using RT-qPCR. d The correlation between LAMC2 expression and primary tumor invasion depth. T1/T2: mucous layer and muscularis propria layer, T3/T4: the adventitia layer and surrounding tissues. e, f ESCC patients with lymph node metastasis and advanced TNM stage showed higher LAMC2 expression. Negative: no lymph node metastasis, positive: with lymph node metastasis. g Kaplan–Meier analysis revealed that high LAMC2 expression predicted poor prognosis in ESCC. h Correlation analysis of LAMC2 and CASC9 expression in ESCC tissues by RT-qPCR. Axis values are transformed by −Log2 of CASC9 expression. *P < 0.05, **P < 0.01, ***P < 0.001

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