Fig. 5

CASC9 promotes ESCC metastasis through regulation of LAMC2 expression. a Transwell assays showed that LAMC2 knockdown attenuated the migration and invasion abilities of KYSE150 and KYSE450 cells. Original magnification, ×100. Scale bars, 200 µm. b Transwell assays showed that LAMC2 overexpression enhanced the migration and invasion abilities of KYSE150 and KYSE450 cells. Original magnification, ×100. Scale bars, 200 µm. c The rescue experiment with Transwell assays was performed in KYSE150 and KYSE450 cells co-transfected with si-NC or si-CASC9-2 and pcDNA3.1 or pcLAMC2. Original magnification, ×100. Scale bars, 200 µm. *P < 0.05, **P < 0.01, ***P < 0.001