Fig. 7 | Cell Death & Differentiation

Fig. 7

From: LncRNA CASC9 promotes esophageal squamous cell carcinoma metastasis through upregulating LAMC2 expression by interacting with the CREB-binding protein

Fig. 7

CASC9 upregulates LAMC2 expression through interacting with CBP. a Subcellular localization of CASC9 and U6 in KYSE150 and KYSE450 detected by RNA FISH. Original magnification, ×630. Scale bars, 20 µm. b, c RT-qPCR and western blot analysis of LAMC2 expression after CBP knockdown in KYSE150 and KYSE450. d Luciferase reporter assay was performed using vector-containing sequences of the LAMC2 promoter region. e RNA pull-down assay showed that CASC9 could retrieve CBP in KYSE150 and KYSE450 cells as detected by western blot. Pull-down indicates the retrieved protein. FT represents the flow-through protein after RNA–protein binding reaction. Input indicates the total cell protein used. f RIP experiment showed that anti-CBP antibody could precipitate CASC9 in KYSE150 and KYSE450 cells. g, h ChIP-qPCR revealed that CASC9 knockdown decreased the enrichment of CBP and the level of H3K27ac acetylation at the promoter region of LAMC2. *P < 0.05, **P < 0.01, ***P < 0.001

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