Fig. 5
SLC25A1 promotes matrix invasion. a–c Representative results showing that CTPI-2 disrupts self-renewal and sphere-forming capacity of the indicated tumor cells relative to monolayer cultures. d, e Spheres embedded in a collagen-based matrix were imaged immediately after plating (time 0) and for 7–10 consecutive days. Fields were identified by numbers on the bottom of the plate. The white rectangles indicate the enhanced areas of each field shown at the top. Invadopodia formation was calculated by first measuring the areas of the spheres (inner area) and the area of the spheres along with its invadopodia (outer area). The percent difference between the outer area and the inner area was graphed and spheres of similar areas were selected for comparison, so to exclude the influence of different cell number. f, g Similar experiments were performed in SLC25A1-expressing cells.Bars represent the standard deviation, asterisks refer to *P ≤ 0.05; ***P ≤ 0.001; ****P ≤ 0.0001 by unpaired T-test
