Fig. 6
SLC25A1 is a key regulator of the citrate pool and redox balance. a Mass spectrometry analysis of citrate in monolayer of sphere cells labeled with [U-13C]glucose. b Assessment of citrate levels in mitochondrial or cytoplasmic fractions and in the presence or absence of CTPI-2, added to the media for four hours before the assay. c Levels of the indicated metabolites assessed with LC-MS in the indicated cell lines grown as spheres. d OCR was assessed in untreated or CTPI-2-treated spheres in the presence or absence of membrane permeable methyl-malate, succinate, or citrate over the course of a 3-h period. e Self-renewal in H1299 cells grown as single cells in semisolid media after 1 week of treatment with the indicated metabolites in mock treated or CTPI-2 treated cells. All metabolites were re-added to the culture media every 48 h. f–h qRT-PCR of the IDH1 mRNA f, assessment of NADP/NADPH ratio g and of superoxide levels detected with mitosox red h, in the indicated conditions. i A Heatmap comparison of the changes in metabolite levels between cells expressing SLC25A1 untreated or treated with CTPI-2 is shown. Asterisks indicate metabolites most relevant to the pathways analyzed in this study
