Fig. 3
Trabid inhibits HCC invasion and lung metastasis by inducing EMT. a The effects of Trabid expression on wound closure in indicated HCC cells. b The effects of Trabid expression on the invasion of HCC cells. Western blotting (c), immunofluorescence (d), and e real-time PCR were used to measure the levels of vimentin, fibronectin, E-cadherin, and ZO-1 expression in the indicated HCC cells. f, g An in vivo lung metastasis analysis. The indicated HCC cell lines were injected into the mice through the tail vein. The metastatic lung foci were counted and detected using bioluminescence imaging. g Representative images of mice lungs and H&E staining (n = 10/group) are shown. h Silencing Trabid prolonged overall survival of xenograft mice models bearing HCCs
