Fig. 7
From: Dpp regulates autophagy-dependent midgut removal and signals to block ecdysone production
Dpp dependent block in ecdysone is not mediated by Dilp8 or Impl2. a Dilp8-GFP expression is undetectable in the midgut and brain complexes with modest GFP detected in eye and wing disc from NP1 > dpp, NP1 > tkvACT. Scale bars, 100 µm. b The level of Impl2 transcripts measured by qRT-PCR from NP1 > dpp and NP1 > tkvACT larvae was increased compared to the control at −4 h RPF. Data are from three experiments, with three larvae per sample (average ± SEM) (**p < 0.01, *p < 0.05 compared to control). c Knockdown of Impl2i (NP1-GAL4/UAS-Impl2RNAi), did not suppress the phenotype of NP1 > dpp and NP1 > tkvACT midguts at −4 h RPF by comparing contraction of the gastric caeca (arrows). Scale bar represents 200 μm. d Quantitation of gastric caeca size in (B) (average pixels ± SD). (**p < 0.001, *p < 0.01 and ns, not significant). e Quantitation by qRT-PCR of the level of RNAi mediated knockdown of Impl2 (average ± SEM)
