Fig. 1

KBM7 FADDˉ cells undergo necroptosis upon treatment with TNFα or the SMAC mimetic birinapant. a Cell viability of KMB7 wildtype and KBM7 FADD- cells treated for 24 h with 100 ng/ml TNFα, 1 µM SMAC mimetic birinapant, 50 µM Nec-1s and 10 µM z-VAD-FMK as indicated. b Cell viability of KBM7 FADD- cells treated for 24 h with 100 ng/ml TNFα, 10 µM SMAC mimetic or 100 ng/ml TNFα and 1 µM SMAC mimetic, and the indicated inhibitors. Cell viability was assessed using a luminescence-based readout for ATP (CellTiter Glo) and normalized to untreated control. Data represent mean value ± s.d. of two independent experiments performed in triplicates