Fig. 7

TNIP1 interferes with TNFα-induced necroptosis and RIPK1 activation in a ubiquitin binding–dependent manner. a, b Cell viability (a) and immunoblot (b) of KBM7 FADD- cells stably expressing the specified V5-tagged TNIP1 constructs or GFP. In a cells were treated as indicated for 24 h and viability was assessed using a luminescence-based readout for ATP (CellTiter Glo). Data represent mean value ± s.d. of two independent experiments performed in triplicates. (c-d) KBM7 FADD- cells stably expressing the specified TNIP1 constructs were stimulated for the time indicated with TNFα (100 ng/ml in c; 10 ng/ml in d). Cells were then lysed and subjected to immunoblotting with the indicated antibodies. Data shown are representative of at least two independent experiments