Fig. 7
From: Novel crosstalk between Vps26a and Nox4 signaling during neurogenesis
Nox4-generated ROS lead to a loss of stemness and subsequent neurogenesis from ESCs. a, b The effects of antioxidant and Nox inhibitor treatments on ROS generation (a) and ESC stemness transcription levels (b) were examined by flow cytometry using WT and Vps26a-/- ESCs differentiated in the presence or absence of 2.5 mM NAC or 10 μM DPI for 6 days. Error bars indicate the means ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001 compared with no treatment. c Double-label immunocytochemical analysis of Vps26a (red), Oct3/4 (green), Nanog (red), and Tubb3 (green) using WT and Vps26a-/- ESCs differentiated in the presence or absence of 20 mM NAC or 2.5 μM DPI for 6 days. DAPI staining data are shown as insets to the Oct3/4 and Tubb3 images. Scale bar, 50 μm. c-i Immunofluorescence quantification of c. Quantification of the fluorescence intensity was performed using ImageJ software (n = 3). Error bars are ± SD. *P < 0.05; ***P < 0.001 vs. WT ND 6 CTL
