Fig. 6 | Cell Death & Differentiation

Fig. 6

From: The transcription factor c-Jun/AP-1 promotes liver fibrosis during non-alcoholic steatohepatitis by regulating Osteopontin expression

Fig. 6

c-Jun promotes Opn expression in NPLCs and the subsequent DR. a Representative immunohistochemical stainings for c-Jun, OPN and CD44 of liver sections from mice with the indicated genotype following 49 days of MCDD. b Hepatic Opn and Cd44 mRNA expression (n = 5–13/genotype and timepoint). mRNA expression is shown relative to untreated controls. c Caspase 3 cleavage was assessed by immunofluorescence (n = 4-10/genotype and timepoint). d Serum ALT concentrations (n = 4–12/genotype and timepoint). e Quantification of Sox9-positive cells by immunohistochemistry (left, n = 5–13/genotype and timepoint). Representative double-immunofluorescence stainings for Sox9 (red) and Opn (green) of livers from mice with the indicated genotype following 49 days of MCDD feeding are shown (right). Double-positive cells are indicated by red arrows. Significance was tested by Mann–Whitney test. P-values are indicated if significant. Scale bar (immunofluorescence) = 25 µm. Scale bar (immunohistochemistry) = 50 µm

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