Fig. 2
Liproxstatin-1 ameliorates ferroptosis and intestinal injury in vivo. Mice were treated with liproxstatin-1 (10 mg/kg) by intraperitoneal injection 1 h before ischemia and then subjected to ischemia/reperfusion (I, 45 min of ischemia; R, 30 min of reperfusion) or sham surgery (sham). All samples were collected after I/R. a GPx4 and COX2 levels were assessed by western blotting (n = 3). b Representative H&E-stained intestinal slices were imaged by microscopy (scale bar = 100μm). c The intestinal permeability was detected by measuring serum FD-4 content (n = 6). d–f 12-HETE, 15-HETE, and LPO were assayed by corresponding kits (n = 6). g–i Serum from mice was used to detect the levels of LDH, TNF-α, and IL-6 (n = 6). All results are expressed as the mean ± SD. **p < 0.01 vs. the sham group; #p < 0.05 vs. the I/R group
