Fig. 1
From: Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas
Brf1 deletion causes a pre-implantation defect. a Strategy used to generate the Brf1flox allele. Grey arrowheads are Flip sites used to excise the puro cassette; black arrowheads are loxP sites inserted flanking exon 3 of the Brf1 locus. b Table of mice and embryos generated after crossing Brf1+/− mice (dpc, days post coitus). c Morphology of 3.5dpc embryos captured with a light microscope. Genotypes are as shown. Percentages depict number of embryos at the blastocyst stage, characterised by vacuole like presence, or the morulae stage. d Top panels show pictures of embryos at 3.5dpc when growth in vitro commenced and after 5 days in culture. Brf1+/+ and Brf1+/− embryos shown after 5 days have colonised the plate. Bottom panel shows graphical representation of the time embryos hatch and rupture the zona pellucida for Brf1+/+ (n = 9) and Brf1+/− (n = 12) embryos. e Top panels show 2.5dpc embryos in vitro at the start of culture and after 2 days in culture. The table shows the genotypes of individual embryos harvested after 2 days in culture
