Fig. 4
From: Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas
Loss of recombined cells upon Brf1 deletion in intestinal crypts. a Immunohistochemistry for RFP on sections of FFPE intestinal tissue. Brf1+/+ mice are a combination of AhCRE Brf1+/+ and AhCre negative mice, whilst Brf1fl/fl mice are AhCre Brf1fl/fl mice. Day post-induction with β-naphthoflavone is indicated above the panels. Scale bar in 100 µm. b BaseScope hybridisation for Brf1 mRNA expression in the intestines of wild-type (induced AhCre negative Brf1fl/fl mice) or Brf1fl/fl mice at 2 and 8dpi. c In situ hybridisation using a probe against tRNAiMET. Brf1+/+ mice are a combination of AhCRE Brf1+/+ and AhCre negative mice, whilst Brf1fl/fl mice are AhCre Brf1fl/fl mice. Day post-induction with β-naphthoflavone is indicated above the panels
