Fig. 5
From: Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas
Brf1 is essential in mouse gut homeostasis. a H&E stained sections of mouse intestinal tissue. Brf1+/+ mice are a combination of AhCRE Brf1+/+ and AhCre negative mice, whilst Brf1fl/fl mice are AhCre Brf1fl/fl mice. Day post-induction with β-naphthoflavone is indicated above the panels. Insets of day 3 samples are 400x magnification to better show tissue morphology. Arrows in the insets show immune cell infiltration and cells undergoing apoptosis. b Top graph shows quantification of cleaved caspase 3 counts. Genotypes are shown on the x-axis and counts per 25 full crypts are shown on the y-axis. Bottom graph shows quantification of apoptotic figures. Genotypes are shown on the x-axis and apoptotic figure counts per 25 full crypts are shown on the y-axis. P-values calculated for a Mann–Whitney non-parametric test are shown. Four mice were used for each genotype. c IHC on sections of intestine from mice harvested 3dpi using the antibodies indicated. Brf1+/+ mice are a combination of AhCRE Brf1+/+ and AhCre negative mice, whilst Brf1fl/fl mice are AhCre Brf1fl/fl mice
