Fig. 4 | Cell Death & Differentiation

Fig. 4

From: Translation of yes-associated protein (YAP) was antagonized by its circular RNA via suppressing the assembly of the translation initiation machinery

Fig. 4

Binding of circYap with linear Yap mRNA. The cell lysates of wide-type MDA-MB231 were incubated with biotinylated scramble oligo, circYap probe (a) or Yap mRNA probe (b) and streptavidin beads. The circYap or Yap mRNA that were pulled down by the probes were examined by real-time PCR. n = 6. **p < 0.01 compared to scramble oligo. c, d Lysates from MDA-MB231 cells transfected with or without vector, circYap or linear Yap mRNA was incubated with circYap or Yap mRNA probe and streptavidin beads. The circYap (c) or Yap mRNA (d) that was pulled down by the probes was examined by real-time PCR. n = 4. **p < 0.01 compared to vector control. e–f Lysates from MDA-MB231 cells were treated with or without RNase R for 15 min at 37 °C followed by incubation with an antibodies against PABP (e) or eIF4G (f) and protein A magnetic beads to precipitate RNAs. The protein-bound RNAs were eluted and the precipitated circYap and Yap mRNA was examined by real-time PCR. n = 6. **p < 0.01 compared to mock (-RNase R)

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