Fig. 7
Role of circYap in tumor cell colony formation, proliferation, and survival. a HepG2 cells were inoculated in 96-well plates to obtain one cell per well. Single cell proliferation was monitored for up to 21 days. n = 50. **p < 0.01 compared to vector control. Days 0–8 are placed as the inset to show the clear difference between vector and circYap. b The HepG2 cells transfected with circYap formed significantly less and smaller colonies than the vector transfected cells. n = 6. **p < 0.01. c Cell proliferation (left) and cell survival (right) were measured in MDA-MB231 cells stably transfected with or without vector, circYap, linYap, or circAmotl1. n = 6. **p < 0.01 compared to vector control. d Cell survival were examined in MDA-MB231 cells transfected with vector, circYap, Mut-1, Mut-2 or Mut-3. n = 6. **p < 0.01 compared to vector control. ##p < 0.01 compared to circYap overexpressed cells. e Cell proliferation (left) and cell survival (right) were measured in MDA-MB231 cells transfected with or without si-ctrl, si-circYap1 and si-circYap2. n = 6. **p < 0.01 compared to siRNA control. f Cell proliferation (left) and cell survival (right) were measured in MDA-MB231 cells transfected with or without si-ctrl, si-Yap1 and si-Yap2. n = 6. **p < 0.01 compared to siRNA control. g Cell proliferation (left) and cell survival (right) were measured in MDA-MB231 cells transfected with or without negative control (NC) or blocking oligos (block-1 and block-2). n = 6. **p < 0.01 compared to negative control
