Fig. 8 | Cell Death & Differentiation

Fig. 8

From: Translation of yes-associated protein (YAP) was antagonized by its circular RNA via suppressing the assembly of the translation initiation machinery

Fig. 8

Role of circYap in tumor cell migration, invasion, and adhesion. a MDA-MB231 cells were stably transfected with vector, circYap, linYap, or circAmotl1. Left, the cells were inoculated in Petri dishes. Adhesive cells were counted 24 h after inoculation. n = 6. **p < 0.01 compared to vector. Middle, cell migration was examined with a scratch migration assay. Five pairs of random points were selected for measuring the migrating distances in each scratch. n = 6. **p < 0.01 compared to vector. Right, MDA-MB231 cells were stably transfected with or without vector, linYap or circAmotl1. Cell invasion was determined by loading 1 × 105 cells in trans-wells with 10% Matrigel in serum-free medium followed by incubation in the 24-well plates with 10%FBS medium during 48 h. n = 6. **p < 0.01 compared to vector. b MDA-MB231 cells were transfected with or without si-ctrl, si-Yap1 or si-Yap2, followed by cell adhesion (left), migration (middle) and invasion (right) assays. n = 6. **p < 0.01 compared to siRNA control. c MDA-MB231 cells were transfected with or without si-ctrl, si-circYap1 or si-circYap2, followed by cell adhesion (left), migration (middle) and invasion (right) assays. n = 6. **p < 0.01 compared to siRNA control. d MDA-MB231 cells were transfected with or without NC, block-1 or block-2, followed by cell adhesion (left), migration (middle) and invasion (right) (right) assays. n = 6. **p < 0.01 compared to negative control

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