Fig. 1
From: Liver stage malaria infection is controlled by host regulators of lipid peroxidation
SLC7a11 signaling is a potent regulator of LS Plasmodium infection. a Schematic of SLC7a11-driven signaling as reported in the literature. b, c Hepa1-6 cells were transduced with 2–4 lentivirus expressing shRNAs against each gene of interest. Cellular lysates were isolated after lentivirus transduction and selection with puromycin and western immunoblots were performed against the indicated targets. Signal was normalized to βActin. d, e Hepa1-6 cells were transduced with lentivirus expressing shRNAs against d GPX4, SLC7a11 or e NOX1 and TFR1 as well as a non-targeting shRNA control (scramble). 1.5 × 105 cells were infected with 5 × 104 P. yoelii sporozoites and quantified by microscopy. f WT or NOX1(−/−) C57Bl/6 mice were infected via retro-orbital injection with 105 P. yoelii sporozoites. Livers were isolated 42 h post-infection and LS burden was quantified by qRT-PCR. g WT or NOX1(−/−) C57Bl/6 mice were perfused and primary hepatocytes from each strain of mouse were isolated. 1.5 × 105 Primary hepatocytes were infected with 105 P. yoelii sporozoites. Infections were quantified by microscopy
