Fig. 2

UBAP2L is required for SG assembly and disassembly. a SG visualization under various stresses or recovery from stresses after UBAP2L knockdown. HeLa cells were transfected with NC- or UBAP2L-siRNA, followed by treatment with or without AS (500 μM, 1 h), H₂O₂ (1 mM, 1 h), or sorbitol (400 mM, 30 min), or recovery from stress for 1 h, and then stained for G3BP (green)/eIF4G (red) as SG markers. b SG visualization under various stresses or recovery from stresses after UBAP2L overexpression. HeLa cells were transfected with Flag tag or Flag-UBAP2L plasmid, followed by the same treatments as in (a), except 100 μM AS for 1 h. c Quantification of SGs in (a). Bars indicate the average percentage of cells containing more than three SGs; between-group differences were analyzed using the chi-square test. d Quantification of SGs in (b). e WB analysis of UBAP2L levels in HeLa cells after UBAP2L knockdown. f WB analysis of UBAP2L levels in HeLa cells after UBAP2L overexpression. Data represent the mean ± SD. *P < 0.05, ***P < 0.001. n = 3. Scale bars = 10 μm