Fig. 2
From: DC-SIGN–LEF1/TCF1–miR-185 feedback loop promotes colorectal cancer invasion and metastasis
DC-SIGN silencing inhibits the growth and metastasis of CRC cells in vitro and in vivo. a Stable CRC cells expressing control shRNA or DC-SIGN shRNA were applied to colony formation analysis. b Growth of subcutaneous xenografts from DC-SIGN shRNA and control cells. c Representative images of LoVo and HCT116 migration and invasion of cells on the membrane. d Fluorescence analysis of the kinetics of metastases of the indicated cells (left panel). The spleens, livers, and lungs were examined at the gross anatomical level for tumors and metastases (middle panel). Representative H&E staining showing metastatic nodules (right panel). T, tumor. e Fluorescent intensity and quantitation of micrometastases per liver and lung as assessed. Data, mean ± SD. *P < 0.05, **P < 0.01. N.D., not determined
