Fig. 5
JMJD6 regulates the level of H4K16ac in cells. a Depletion of JMJD6 leads to increased H4K16ac level. Total proteins from U2OS cells transfected with the control or JMJD6 siRNAs were extracted, and western blot analysis was performed using antibodies against the indicated proteins. b JMJD6 knockdown does not increase the total level of methylation on H4R3 or H3R2. Total proteins from U2OS cells transfected with the control or JMJD6 siRNAs were extracted, and western blot analysis was performed using antibodies against the indicated proteins. c JMJD6 depletion does not change the mRNA levels of TIP60, MOF, or SIRT1. Total mRNA from U2OS cells transfected with indicated siRNAs was extracted, and quantitative real-time RT-PCR assays were performed. Each bar represents the mean ± S.D. for triplicate experiments. d JMJD6 knockdown does not change the protein level of TIP60, MOF, or SIRT1. e ChIP-seq density heatmaps of JMJD6 and H4K16ac around 6292 JMJD6-binding peaks in HeLa cells. f ChIP-seq profiling of JMJD6 and H4K16ac in HeLa cells over a 1112-bp window. g Knockdown of JMJD6 increases digestion by micrococcal nuclease. Nucleosomes from control or JMJD6-depleted U2OS cells were digested by micrococcal nuclease and then subjected to DNA gel electrophoresis
