Fig. 3
From: T-cell receptor signal strength and epigenetic control of Bim predict memory CD8+ T-cell fate
DNA methylation represses Bim expression. a A CpG condensed region, CpG island 256, covering the promoter, transcription start site, and 5ʹ-UTR of Bcl2l11 gene was identified. b Frequencies of methylated CpG sites in the CpG island 256. Endogenous TCM or TEM cells from uninfected C57BL/6 mice were sorted, and the promoter region (−461/ + 4) was analyzed by bisulfite sequencing. Twenty clones from each population were tested. c MFI of Bim ICS from splenic CD8+ T cells in vitro cultured with 5-aza-2’-deoxycytidine and IL-2 (20 ng/ml) for 3 days. (Mean ± SD, *p < 0.05, **p < 0.01, comparing to untreated Ctrl. Unpaired two-tailed Student’s t-test). Results are representative of two independent experiments
