Fig. 4

Nur77^hi cells have high levels of Bim, high TCR affinity, and bias to pre-memory cell and T_CM differentiation. a Splenic CD8⁺ T cells were harvested from Bim^mCherryNur77^GFP double-reporter mice with LCMV infection on day 10. H-2D^b-GP33 tetramer⁺ CD8⁺ T cells were analyzed by flow cytometry. The Bim^mCherry MFI of CD8⁺ T cells with the top or the bottom 10% Nur77^GFP expression are shown (mean ± SD, ** p < 0.01, paired two-tailed Student’s t-test). b The correlation between mCherry and GFP MFI are shown in Pearson r and p-value. The flow plot is representative of four mice and repeated in two independent experiments. c Bar graphs (mean ± SD) show frequencies of terminal effector cells, pre-memory cells, or d T_CM cells among transferred Nur77^hi (black bars) or Nur77^lo (white bars) CD8⁺ cells on 24 dpi (n = 4, ***p < 0.001 unpaired two-tailed Student’s t-test). e Splenic CD8⁺ T cells were harvested from Nur77^GFP mice with LCMV infection on day 10. H-2D^b-GP33 tetramer⁺ CD8⁺ T cells were stained with TCRβ Ab. The MFI ratio (tetramer/TCRβ) of cells with the top or the bottom 10% Nur77^GFP MFI were calculated and shown. Results are representative of two independent experiments. (*p < 0.05, paired two-tailed Student’s t-test)