Fig. 2

Hepatic G9a deletion augments LPS-induced inflammation, RNS/ROS production, DNA damage and hepatocyte apoptosis. a Representative pictures for F4/80, Ly-6G and CD3 staining in the liver (left) and quantitative results (right). b qPCR results of the indicated genes in the liver of different groups. c, d Representative western blots (left) and quantitative results (right) for VCAM-1, ICAM-1, and MCP-1 (c), iNOS, eNOS and 3-NT (d) in livers of different groups. HSP70 serves as the loading control. e Representative pictures for 3-NT staining in the liver (left) and quantitative results (right). f Relative mRNA level of Atf3 in the liver at 1 h after LPS injection. g Representative pictures for 8-oxoG staining in the liver (left) and quantitative results (right). n = 3–4 per group; 3-NT, 3-Nitrotyrosine; n.s., not significant; ^*p < 0.05; scale bar, 50 μm