Fig. 2

TXNDC12 enhances the metastatic potential of HCC cells. a Overexpression of TXNDC12 in Hep3B cells and knockdown of TXNDC12 in HCCLM3 cells, as confirmed by immunoblot analysis. b Representative data from wound healing migration assays performed with the indicated HCC cells. Scale bar = 200 μm. The data are the means ± SDs and are representative of three independent experiments. c Representative data from Transwell migration and Matrigel invasion assays performed with the indicated HCC cells. Scale bar = 200 μm. The data are the means ± SDs and are representative of three independent experiments. d Representative data from the cell adhesion assay performed with the indicated HCC cells. The data are the means ± SDs and are representative of three independent experiments. e Representative images (gross, HE, and IHC of TXNDC12) and the quantification of liver metastasis nodules, as well as the tumoral expression of TXNDC12, from the orthotopic HCC models established by injection of the indicated cells (n = 6 per group). f Representative images (gross, HE, and IHC of TXNDC12) and quantification of lung metastasis nodules, as well as the tumoral expression of TXNDC12, from the lung metastasis models established by injection of the indicated cells (n = 6 per group). *P < 0.05; **P < 0.01; ***P < 0.001