Fig. 6
From: TXNDC12 promotes EMT and metastasis of hepatocellular carcinoma cells via activation of β-catenin
Mutation of the cysteines in the active site of TXNDC12 blocks its interaction with β-catenin and inhibits β-catenin activation. a 293T cells transfected with Flag-tagged vector, wild-type TXNDC12 (WT), or cysteine-mutated TXNDC12 (CS) were immunoprecipitated with an anti-Flag antibody. The β-catenin and Flag-TXNDC12 (full-length or fragment) levels are indicated. b β-Catenin expression in the indicated cells, as detected by immunofluorescence assay. The merged images show overlays of β-catenin (red) and nuclear staining by DAPI (blue); scale bar: 20 mm. c TOP-Flash/FOP-Flash assay showing β-catenin activity in the indicated HCC cells. d Relative expression levels of TXNDC12, ZEB1, E-cadherin, and Vimentin in the indicated cells. e Wound healing migration assays performed with the indicated HCC cells. The data are the means ± SDs and are representative of three independent experiments. f Transwell migration and Matrigel invasion assays performed with the indicated HCC cells. The data are the means ± SDs and are representative of three independent experiments. g Representative data from the cell adhesion assay performed with the indicated HCC cells. The data are the means ± SDs and are representative of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001
