Fig. 5
ERK/MAPK signaling pathway is specifically activated by AMG treatment. a Representative western blotting out of three biological replicates showing protein level of phosphorylated and total ERK1/ERK2 from wounded fibroblasts upon AMG treatment. b Representative images of scratch wound assays. Unprocessed AMG and its soluble fraction (SF) were applied on wounded cells. Each condition was also incubated with the MEK inhibitor—PD0325901—1 μM for the same time periods of the AMG treatment. Wounded cells did not receive any treatment and were used as a control. Scale bar = 200 μm. c Quantifications of AMG-treated cells with or without PD0325901 for each time of treatment are reported. Data are presented as mean ± SEM. Significant differences vs control were calculated with multiple unpaired t test (N = 3) and indicated as *P < 0.05. d MMP gene expression evaluated by RT-qPCR upon AMG treatment and MEK inhibitor PD0325901 exposition. Expression values are expressed as a -∆CT normalized on the expression of Gapdh, B-actin, and Rpl13a housekeeping genes. Differences are calculated with one-way ANOVA (N = 3) and indicated as *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
