Fig. 3: Downregulation of ENaC levels after high-Na⁺ diet is compromised in Nedd4-2^Ksp1.3 kidneys.

a Semi-quantitative immunoblot analysis reveals decreased expression of mature ENaC in control and Nedd4-2^Ksp1.3 (N42^Ksp1.3) kidneys after a high-Na⁺ diet, however, levels remain elevated in Nedd4-2^Ksp1.3 kidneys. Expression levels quantitated in b as fold change from control standard diet, relative to β-actin expression. c Immunohistochemical staining of ENaC subunits reveals increased membrane localisation in Nedd4-2^Ksp1.3 kidneys. High Na⁺ results in reduced membrane α-ENaC in control kidneys, but not in Nedd4-2^Ksp1.3 kidneys. Scale bar: 50 μm. n = 3, mean ± SEM with significance calculated by a Student’s t test (2 tailed). *P < 0.05, ***P < 0.005.