Fig. 3: Overexpression of TRIM22 promotes proliferation of GBM in vitro and in vivo via its RING domain. | Cell Death & Differentiation

Fig. 3: Overexpression of TRIM22 promotes proliferation of GBM in vitro and in vivo via its RING domain.

From: TRIM22 activates NF-κB signaling in glioblastoma by accelerating the degradation of IκBα

Fig. 3

a Western blot analysis to evaluate components of the NF-κB pathway in lysates prepared from LN229- and U118MG-EV, TRIM22-FL and TRIM22-ΔRING cell populations. GAPDH was used as the loading control. b Growth curves generated using cell counting and c Ki-67 immunofluorescence staining for transfected cells in a. Scale bars, 100 μm. d, e Representative images and quantification of in vivo luciferase bioluminescence from indicated cells orthotopically implanted into the brains of nude mice at day 6, 12, and 24 after injection. f Kaplan–Meier analysis of survival for tumor-bearing mice implanted with indicated cells. Log-rank test, P < 0.05. Student’s t test: n.s. not significant, *P < 0.05, **P < 0.01.

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