Fig. 7: KPNB1 is responsible for PD-L1 nuclear translocation. | Cell Death & Differentiation

Fig. 7: KPNB1 is responsible for PD-L1 nuclear translocation.

From: KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway

Fig. 7

a The mass spectrometry assay of the potential PD-L1-interacting proteins. b Endogenous KPNB1 and PD-L1 interaction were detected in NSCLC cells using anti-KPNB1 and anti-PD-L1 antibodies for co-IP and Western blot assays. c HEK293T and Hela cells were transfected with the indicated plasmids. Western blot and co-IP assays were performed to assess the interaction between PD-L1 and KPNB1. d The cellular location of PD-L1 and KPNB1 examined by confocal microscopy (Scale bar: 5 μm). e Correlation assay of PD-L1 and KPNB1 expression in NSCLC patient samples based on linkedomics and GEPIA database. f, h Western blot assay of cytoplasmic and nuclear PD-L1 expression after KPNB1 knockdown with two specific siRNAs or knockout with CRISPR-Cas9 gene editing system. g Immunofluorescence staining of nPD-L1 location after KPNB1 knockdown in PD-L1-overexpressed cells (Scale bar: 5 μm). i Western blot assay of nPD-L1 expression in KPNB1-overexpressed cells.

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